Acrylamide gel electrophoresis studies of the incorporation of cytidine- 3 H into mouse skin RNA at early times after treatment with phorbol esters.

The nature of the early RNA response was analyzed by polyacrylamide gel electrophoresis. The extraction procedure allowed quantitative isolation of undegraded RNA from whole mouse skin. By 3 hr after treatment with effective tumor promoters, there was a large stimulation of incorporation of cytidine-3H into "32 S" RNA, rRNA, and 4 to 5 S RNA. Stimulation of cytidine-3 H incorporation into 32 S RNA was